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  • Rapid detection of E. coli cells in urine samples using a ...

    Escherichia coli is one of the main causes of urinary tract infections (UTIs). E. coli is commonly detected from urine using standard culture method. However, the urine sampling and analysis required for these methods can be costly, time consuming (requires 24 to 48 hours) and labor-intensive. This …

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  • Impedimetric method for measuring ultra-low E. coli ...

    In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis. E. coli growth in human urine samples was successfully monitored during a …

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  • A multiplex PCR method to detect 14 Escherichia coli ...

    E. coli strains belonging to 14 serogroups, including O1, O2, O4, O6, O7, O8, O15, O16, O18, O21, O22, O25, O75 and O83, are the most frequently detected UPEC strains in a diverse range of clinical urine specimens. In the current study, the O-antigen gene clusters of E. coli serogroups O1, O2, O18 and O75 were characterized.

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  • Impedimetric method for measuring ultra-low E. coli ...

    In this study, we developed an interdigitated gold microelectrode-based impedance sensor to detect Escherichia coli (E. coli) in human urine samples for urinary tract infection (UTI) diagnosis.E. coli growth in human urine samples was successfully monitored during a 12-h culture, and the results showed that the maximum relative changes could be measured at 10 Hz.

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  • Development of a fluorescent distance-based paper device ...

    2020-10-1 · The highly sensitive and selective determination of Escherichia coli (E. coli) in urine was achieved using a SYBR™ safe loop-mediated isothermal amplification (LAMP) method with a distance-based paper device.New primers set specific to multi-copy the 16s rRNA gene of E. coli were designed and used in this study. The detection sensitivity of these primers was higher than …

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  • Recent advances in biosensor based diagnosis of urinary ...

    2016-6-15 · A 96 well plate is the commonly used where the first wells are used to draw the standard curve. The samples are added in duplicate or triplicate and then the mean results are calculated. A paper-based ELISA has been developed recently that can detect E. coli or bacteriuria in 5 h (Shih et al., 2015).

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  • how do you get e-coli in your urine? | Answers from ...

    2017-9-25 · Travel up urethra: E. Coli species are the most common bowel bacteria and are invariably present on the perineal skin, no matter how well you wipe. They will be present in any urine temporarily trapped behind the labia and get 'sucked' up the urethra during terminal muscle contractions at end of voiding. E. Coli around the urethral opening can be pushed up the urethra …

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  • A Universal Method for the Identification of Bacteria ...

    2011-2-19 · Mathematically, the two pairs QUGP-F3.R2 and QUGP-F5.R4 will detect (95.4%) all 44 but (Chlamydophila1 and Mycoplasma). However, mixing the two pairs will have the added advantage of generating a new pair (QUGP-F5.R2) which will detect Mycoplasma but not Chlamydophila1. Therefore mixing the four primers will result in the detection of 43 genera …

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  • Recent advances in biosensor based endotoxin detection ...

    2014-1-15 · A disposable amperometric immunosensing strip was prepared to rapidly detect E Coli O157:H7 with lower detection limit of 6 CFU/strip in PBS buffer and 50 CFU/strip in milk (Lin et al., 2008). A new amendment to the regular amperometric sensor using bienzyme sensor has been developed for detection of E. coli .

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  • Wakana OISHI | Tohoku University, Sendai | Tohokudai ...

    This study proposed an inactivation kinetic model of Escherichia coli (E. coli), a surrogate of human bacterial pathogens, in concentrated synthetic urine. The model parameters were obtained under ...

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  • A Universal Method for the Identification of Bacteria ...

    2011-2-19 · A robust method, required for bacterial identification, has been perused by several investigators [4, 14, 26, 36–40]; studies on universal and multiplex primers].With the current number of eubacterial species surpassing 7166 species [], the UM described here [] should fulfil this requirement.The UM had integrated several general primers, PCR amplification, DNA …

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  • Microbiological testing of water

    The IDEXX Colilert uses a colourimetric ONPG assay to detect coliforms and a fluorescence MUG assay for E. coli. Colilert can simultaneously detect these bacteria within 18-24 hours, depending on the product. It can also suppress 2 million heterotrophic bacteria per 100 mL present.

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  • Frontiers | Modern Tools for Rapid Diagnostics of ...

    2020-7-15 · It can detect bacterial growth in 3 h from clinical urine samples containing >10 4 cfu/ml of E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus antracis, Yersinia pestis, or Burkholderia pseudomallei (Bugrysheva et al., 2016; Hayden et al., 2016; Montgomery et al., 2017). The BacterioScan 216R Rapid AST System is currently under ...

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  • Analytix - Sigma-Aldrich

    2016-10-29 · from clinical material like stool, urine, skin scurf and swabs. E. coli 0157:H7 HiCrome™ Enrichment Broth Base for EC O157: H7 Fluka 80330 Recommended for isolation and selective differentiation of E. coli O157:H7 from food and environmental samples by chromogenic method. E. coli (thermotolerant)

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  • Wakana OISHI | Tohoku University, Sendai | Tohokudai ...

    This study proposed an inactivation kinetic model of Escherichia coli (E. coli), a surrogate of human bacterial pathogens, in concentrated synthetic urine. The model parameters were obtained under ...

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  • Scientists Hacked E. coli Into Producing A Powerful ...

    2021-4-15 · The LOD for E. coli O157:H7 and Salmonella using this approach was 1–10 CFU mL −1 in drinking water (C. Wang et al., 2017). Similarly, the Banerjee lab at Alabama A&M University combined a SERS molecular probe with magnetic-separation (label assay), showing a LOD of 10 2 CFU mL −1 for E. coli O157:H7 in apple juice (Najafi et al. (2014).

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  • FEAST of biosensors: Food, environmental and

    2021-2-17 · Synthesis of Au microwires. Au microwires were synthesised based on the method described in the literature [].In the absence of heat, the synthesis reaction involved mixing of 100 µL OA and 150 µL TIPS with about 3 to 5 mg of gold (III) chloride precursor solution, (HAuCl 4 99.9% trace metals basis, 30 wt% in dilute HCl) in 2.5 mL final volume of hexane without stirring.

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  • Synthesis, characterisation and cytotoxicity of gold ...

    2019-3-1 · Carbon dots (CDs), also called carbon quantum dots, make up a new class of fluorescent carbon nanomaterials with ultrafine sizes below 10 nm. Since CDs were first discovered in 2004 [1] during the separation and purification of single-walled carbon nanotubes, the properties, synthesis and applications of CDs have been widely researched ( Fig. 1 ).

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  • Fast detection of E. coli with a novel fluorescent ...

    Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and [email protected] 3 O 4 in urine specimens Y. Yao, G. Xie, X. Zhang, J. Yuan, Y. Hou and H. Chen, Anal. Methods, 2021, 13, 2209 DOI: 10.1039/D1AY00320H

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  • Often asked: How can you get e coli in your urine?

    2021-3-15 · What does it mean to have E coli in your urine? Infection of the bladder (cystitis).. This type of UTI is usually caused by Escherichia coli (E.coli), a type of bacteria commonly found in the gastrointestinal (GI) tract. However, sometimes other bacteria are responsible. Sexual intercourse may lead to cystitis, but you don’t have to be sexually active to develop it.

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  • Microbiological testing of water

    2021-4-27 · Fast detection of E. coli with a novel fluorescent biosensor based on a FRET system between UCNPs and [email protected] in urine Analytical Methods ( IF 2.896) Pub Date : 2021-4-6, DOI: Yuan Yao, Guoming Xie, Xin Zhang, Jinshan Yuan, Yulei Hou, Hui Chen

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  • Laboratory Diagnosis of Escherichia coli Infections

    The IDEXX Colilert uses a colourimetric ONPG assay to detect coliforms and a fluorescence MUG assay for E. coli. Colilert can simultaneously detect these bacteria within 18-24 hours, depending on the product. It can also suppress 2 million heterotrophic bacteria per 100 mL present.

    Get Price
  • Reduction in energy for electrochemical disinfection of E ...

    Laboratory Diagnosis. Laboratory diagnosis of E. coli infections is based on. a) Isolation of E. coli by culture. b) Demonstration of toxins of diarrheagenic E. coli. Specimens Urine is the specimen of choice for diagnosis of UTI caused by uropathogenic E. coli.Clean-voided midstream samples of urine are usually employed for culture.

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  • Urinalysis Parameters - News-Medical.net

    2019-3-6 · We report the development of novel modes of operation for electrochemical disinfection of E. coli in human urine simulant with an aim to minimize the energy required for disinfection. The system employs boron-doped diamond electrodes and will be part of an energy neutral, water and additive free outdoor toilet being developed for use in developing countries.

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  • Urine tTest Strips,Water Test Strips,Rapid Test Kit,HCG,LH ...

    Export to Europe, America, and Asia, reasonable price, quality assurance, timely and effective pre-sale, after-sale service, products can be in supermarkets, pharmacies, and hospitals to sell and use, the company can provide relevant qualifications. Our urine test paper can detect 14 substances in the urine, water test paper can detect 39 sub...

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  • i have e.coli in urinary tract. what does that mean ...

    E coli is bacteria that caused strap infection and when the infection is severe you can get the bacteria in your urine as well. The good thing is that you can treat this bacteria with antibiotics. You should ask your doctor about prescription for antibiotics and see if …

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  • Multiplex detection of bacteria on an integrated ...

    2017-5-3 · Further, LoD for E. coli observed during analysis by our method was similar to that reported by another LAMP-based centrifugal chip 44 (2.7 × 10 4 CFU per 1 mL sample). However, this chip is not ...

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  • MicroSnap E. coli - Indicator Organisms - Hygiena

    2021-7-10 · MicroSnap™ E. coli is a rapid test for detection and enumeration of E. coli bacteria.The test uses a novel bioluminogenic test reaction that generates light when enzymes that are characteristic of E. coli bacteria react with specialized substrates. The light-generating signal is then quantified in the EnSURE™ Touch luminometer.

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  • How is E. coli Infection Diagnosed? | About E. coli

    For many years, the only test was a stool culture, a type of test that could readily detect E. coli O157:H7 because its unique growth characteristics could be used to differentiate it from most other types of E. coli, including the many harmless strains that commonly inhabit the human intestine.

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  • First Instant Test to Detect Presence of E. Coli 1057:H7

    2021-2-13 · 1. Introduction. Escherichia coli, a Gram-negative bacterium commonly found in the intestinal flora of humans and animals, is the etiological agent responsible for a variety of common infectious diseases, including urinary tract infections [] and gastroenteritis [].There is a need for rapid, timely, and specific diagnostic procedures to detect E. coli infection.

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  • Turntable Paper-Based Device to Detect Escherichia coli

    2017-9-25 · Travel up urethra: E. Coli species are the most common bowel bacteria and are invariably present on the perineal skin, no matter how well you wipe. They will be present in any urine temporarily trapped behind the labia and get 'sucked' up the urethra during terminal muscle contractions at end of voiding. E. Coli around the urethral opening can be pushed up the urethra …

    Get Price
  • how do you get e-coli in your urine? | Answers from ...

    2021-4-30 · This study aimed to detect carbapenem-resistant blaNDM-1 gene in ESBL producing E. coli and K. pneumoniae isolates. Methodology A total of 190 E. coli and 350 K. pneumoniae isolates were screened for extended spectrumβ-lactamase (ESBL), carbapenemase and metallo β-lactamase (MBL) production via double-disk synergy test (DDST), modified Hodge ...

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  • Detection of blaNDM-1 gene in ESBL producing Escherichia ...

    The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities. 'The novelty of this test is that it targets four genes,' Nagaraja said. 'We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces.' To ...

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  • Researchers Create Better Methods to Detect E. coli | Lab ...

    Antibiotic prescribing following urine dipstick screening has been a widely adopted management approach for UTIs with one study showing this to be cost-effective if the value of saving a day of moderately bad symptoms is valued at ≥ £10. 22 However, this practice is also known to be a major contributor to the emergence of multi-drug ...

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  • Point-of-care testing for urinary tract infections — NIHR ...

    2018-3-22 · As strain variation and drug resistance become more pervasive, the prevention and control of infection have been a serious problem in recent years. The detection of pathogen is one of the most important parts of the process of diagnosis. Having a series of advantages, such as rapid response, high sensitivity, ease of use, and low cost, biosensors have received much …

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  • Recent advances in rapid pathogen detection method based ...

    Efficient product and energy saving strategies can help us to save money and energy at home. The idea of conserving electricity means you should only use it when necessary and avoid wasting it.

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  • E. coli (Escherichia coli) | E. coli | CDC

    Escherichia coli (abbreviated as E. coli) are bacteria found in the environment, foods, and intestines of people and animals.E. coli are a large and diverse group of bacteria. Although most strains of E. coli are harmless, others can make you sick. Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses.

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  • 9 Methods to Identify and Detect Microbial Contaminants in ...

    2021-7-9 · For example, Pyle et al. (1995) combined incubation using cyanoditolyl tetrazolium chloride (CTC) to detect respiratory activity with a modified FA technique, for the enumeration of viable E. coli O157:H7 in water in three to four hours. Bacteria were captured by filtration on nonfluorescent polycarbonate membranes, incubated on absorbent pads ...

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  • Chickenpox (Varicella) | Interpreting Laboratory Tests | CDC

    Laboratory Testing. Laboratory testing is recommended to: Confirm suspected cases of varicella. Confirm varicella as the cause of outbreaks. Confirm varicella in severe cases (hospitalizations or deaths) or unusual cases. Determine susceptibility to varicella. Determine if suspected vaccine-related adverse events were caused by vaccine-strain VZV.

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  • Wearable Health Devices—Vital Sign Monitoring, Systems

    2018-7-25 · 1. Introduction. Wearable Health Devices (WHDs) are an emerging technology that enables continuous ambulatory monitoring of human vital signs during daily life (during work, at home, during sport activities, etc.) or in a clinical environment, with the advantage of minimizing discomfort and interference with normal human activities [].WHDs are part of personal health …

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  • BBL Family of Products Delivering Efficiency in Living Color

    2021-7-10 · S t a p h y l o c o c c u s s u rv e i l l a n c e .1Additional benefits include: •Isolates and identifies Staphylococcus aure u sf rom clinical sources without the use of confirm a t o ry testing.2 •Shows increased re c o v e ry by 11% when compared to Mannitol Salt A g a r, as demonstrated in a recent clinical study.1

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  • Current and Future Perspectives on Isothermal Nucleic Acid ...

    2019-10-19 · An example is the BD ProbeTec TM ET system designed for detecting Chlamydia trachomatis in urine 145,151 and Neisseria gonorrhoea in urine. 152 Clinical studies on patient urine and swab samples with the developed SDA-based assay reported a sensitivity of 100% and specificity of 100% for detection of C. trachomatis and N. gonorrhea nucleic acid ...

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  • Application of Electrochemical Methods in Biosensing ...

    2017-12-20 · Introducing biochemical factor to electronic devices have created a new branch of science. Recent development in biosensing technology enabled progress in pathogens detection. Currently, wide range of biomarkers (enzymes, peptides, DNA, microorganisms, etc. )recognize various target analytes, starting from basic metabolism changes to serious infections caused by …

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  • Detection of DNA fusion junctions for BCR - ABL ...

    2010-9-22 · Anchored ChromPET, a technique to capture and interrogate targeted sequences in the genome, has been developed to identify chromosomal aberrations and define breakpoints. Using this method, we could define the BCR-ABL1 translocation DNA breakpoint to a base-pair resolution in Philadelphia chromosome-positive samples. This DNA-based method is highly sensitive and can detect …

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  • Patenting inventions arising from biological research ...

    2004-12-20 · Time and money spent at this stage is well spent, because the results of the search can guide applicants in both preparing the patent application and entering the marketplace with a viable product. Numerous free and commercial public databases are available for searching: a list of some commonly used ones is shown in Table 2.

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  • A qPCR method for genome editing efficiency determination ...

    2019-12-11 · CRISPR/Cas9 technology has been widely used for targeted genome modification both in vivo and in vitro. However, an effective method for evaluating genome editing efficiency and screening single ...

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